Fetal Bovine Serum from MilliporeSigma

Product Specs
ItemFetal Bovine Serum
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberF4135
Speciesbovine fetus
Applicationsinsect cell culture, hybridoma cell culture, mammalian cell culture
Quantity100ML, 1L, 24X500ML, 500ML, 6X500ML

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

Company Profile

Website: www.SigmaAldrich.com/

(1) Human Hepatocellular response in Cholestatic Liver DiseasesOrganogenesisDecember 31, 2023Kimberly Ortiz, Zeliha Cetin, Yiyue Sun, Zhiping Hu, Takeshi Kurihara, Edgar N. Tafaleng, Rodrigo M. Florentino, Alina Ostrowska, Alejandro Soto-Gutierrez, Lanuza A.P. FaccioliLeibovitz’s L-15 medium (cat. 21-083-027, GIBCOTM) supplemented with 5% of fetal bovine serum (cat. F4135, Sigma Aldrich), and filtered through a 215 and 125 µm mesh. The final crude cell suspensions were centrifuged

(2) Cytokine induced inflammatory bowel disease model using organ-on-a-chip technologyPLoS OneDecember 13, 2023Christine Tataru, Maya Livni, Carrie Marean-Reardon, Maria Clara Franco, Maude Davidmedia bullet kit,Lonza,CC-3162 Matrigel,VWR,47743–715 Type1 Collagen,VWR,47747–218 FBS,Sigma-Aldrich,F4135 Trypsin-EDTA,Sigma-Aldrich,T3924 Cascade Blue fluorescent salt,ThermoFisher Scientific,C687 IL-8

(3) ARID1A loss activates MAPK signaling via DUSP4 downregulationJ Biomed SciDecember 9, 2023Jayaprakash Mandal, Zheng-Cheng Yu, Ie-Ming Shih, Tian-Li WangThese hEM3 cells were kept in RPMI 1460 medium (Gibco, 11875093) supplemented with 15% FBS (Sigma, F4135), 1% Pen/Strep, and 1% NEAA. For experimental purposes, the hEM3 cells were moved to RPMI containing

(4) In Vitro Evidence of Selective Pro-Apoptotic Action of the Pure Cannabidiol and Cannabidiol-Rich ExtractMoleculesDecember 1, 2023Paweł Śledziński, Agnieszka Nowak-Terpiłowska, Piotr Rzymski, Ryszard Słomski, Joanna ZeylandGermany) and nutrient mixture F-12 (N6658, Sigma-Aldrich), supplemented with 10% (v/v) fetal bovine serum (F4135, Sigma-Aldrich) and 1% (v/v) penicillin/streptomycin (P4333, Sigma-Aldrich). Cells were maintained at

(5) Cytotoxic pathways activated by multifunctional thiosemicarbazones targeting sigma-2 receptors in breast and lung carcinoma cellsPharmacological ReportsDecember 1, 2023Joanna Kopecka, Alessandra Barbanente, Daniele Vitone, Fabio Arnesano, Nicola Margiotta, Paola Berchialla, Mauro Niso, Chiara Riganti, Carmen AbateScientific; # 61,870,010), all supplemented with 10% v/v fetal bovine serum (FBS; Sigma, St. Louis, MO; #F4135) and 1% v/v penicillin–streptomycin (Sigma; #P4458). MCF10A cells were cultured in DMEM/F12 medium (Gibco

(6) METTL3 alters capping enzyme expression and its activity on ribosomal proteinsbioRxivNovember 26, 2023Daniel del Valle-Morales, Giulia Romano, Patricia Le, Michela Saviana, Rachel Brown, Lavender Micalo, Howard Li, Alessandro La La Ferlita, Giovanni Nigita, Patrick Nana-Sinkam, et al.cell lines were seeded and grown in RPMI (Sigma-Aldrich #R8758), supplemented with 10% FBS (Sigma #F4135) and 1% Penicillin/Streptomycin (Quality Biological #120-095-721). Stable A549 cell lines were generated

(7) Modulation of SLFN11 induces changes in DNA Damage response in breast cancerCancer Cell InternationalNovember 24, 2023Christophe Michel Raynaud, Eiman I. Ahmed, Ayesha Jabeen, Apryl Sanchez, Shimaa Sherif, Tatiana C. Carneiro-Lobo, Amany Awad, Dina Awartani, Adviti Naik, Remy Thomas, et al.All cell lines were cultured in advanced RPMI (Gibco, #12633012) complemented with 10% FBS (Sigma, #F4135), Glutamax (Gibco, #35050061) and antibiotic–antimycotic (Gibco, #15240096). Cells were cultured at

(8) Physical activity of mice on dietary sulfur amino acid restriction is influenced by age of diet initiation and biological sexScientific ReportsNovember 23, 2023Diana Cooke, Gene P. AblesLonza #12604Q, Walkersville, MD, USA) containing 10% heat-inactivated fetal bovine serum (FBS, Sigma #F4135), and 1% penicillin/streptomycin (Gibco Life Technologies #15070063) at 37 °C in a humidified atmosphere

(9) Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissuesiScienceNovember 17, 2023Francis M. Barnieh, Sebastian P. Galuska, Paul M. Loadman, Simon Ward, Robert A. Falconer, Sherif F. El-Khamisyand recombinant proteins" ,, RPMI-1640,Merck-UK,Cat# R0883-500ML Fetal Bovine Serum,Merck-UK,Cat# F4135-500ML Phosphate buffered saline,Merck-UK,Cat# P2272-500ML "α2-3,6,8 Neuraminidase",New England Biolabs,Cat#

(10) Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosisFrontiers in ImmunologyNovember 16, 2023Qiuhua Yang, Emily Huo, Yongfeng Cai, Zhidan Zhang, Charles Dong, John M. Asara, Huidong Shi, Qingqing Weicultured in RPMI 1640 medium (SH30027.01, Cytiva, Marlborough, MA, USA) supplemented with 10% FBS (F4135, Sigma-Aldrich, St. Louis, MO, USA), 10 ng/mL MCSF (315-02, PeproTech, Cranbury, NJ, USA) and 1% Antibiotic-Antimycotic

(11) Identification of Host Factors for Rift Valley Fever PhlebovirusVirusesNovember 13, 2023Velmurugan Balaraman, Sabarish V. Indran, Yonghai Li, David A. Meekins, Laxmi U. M. R. Jakkula, Heidi Liu, Micheal P. Hays, Jayme A. Souza-Neto, Natasha N. Gaudreault, Philip R. Hardwidge, et al.L-15 medium (ATCC, USA), supplemented with 10% insect cell culture tested FBS (IFBS, Catalog Number: F4135, Sigma-Aldrich, St. Louis, MO, USA), 10% tryptose phosphate broth (TPB, Catalog Number: T9157, Sigma-Aldrich,

(12) Distinct Patterns of Gene Expression Changes in the Colon and Striatum of Young Mice Overexpressing Alpha-Synuclein Support Parkinson’s Disease as a Multi-System ProcessJournal of Parkinson's DiseaseNovember 3, 2023Elizabeth J. Videlock, Asa Hatami, Chunni Zhu, Riki Kawaguchi, Han Chen, Tasnin Khan, Ashwaq Hamid Salem Yehya, Linsey Stiles, Swapna Joshi, Jill M. Hoffman, et al.conical tube containing 30 ml cold HBSS and vortexed with eight 5-s pulses. Fetal Bovine Serum (Sigma F4135, St Louis, MO) was added to a final concentration of 10% and the solution was centrifuged at 125 g for

(13) Research Note: Injection of adenoviral CRISPR/Cas9 system targeting melanophilin gene into different sites of embryos induced regional feather color changes in posthatch quailPoultry ScienceNovember 1, 2023Joonbum Lee, Dong-Hwan Kim, Kichoon Leemodified Eagle's medium (DMEM, #11965, Gibco, Grand Island, NY) with 10% fetal bovine serum (FBS, #F4135, Sigma-Aldrich, St. Louis, MO) and penicillin-streptomycin solution (100 U/mL, #15140, Gibco) for 2

(14) Methods for cell isolation and analysis of the highly regenerative tunicate Polycarpa mytiligeraFrontiers in Cell and Developmental BiologyOctober 11, 2023Tal Gordon, Noam Hendin, Omri Wurtzelwere resuspended in 800 µL of L-15 medium containing glucose and Fetal Bovine Serum (Sigma Aldrich, F4135) in a 24-well plate. For the treatment groups, the medium was supplemented with 8 μL of liposomes containing

(15) Signaling dynamics distinguish high- and low-priority neutrophil chemoattractant receptorsScience SignalingOctober 3, 2023Stefan M. Lundgren, Briana L. Rocha-Gregg, Emel Akdoğan, Maya N. Mysore, Samantha Hayes, Sean R. Collinscatalog #72–400-120) media with 9% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, catalog #F4135) and 100 U/ml penicillin-100 mg/ml streptomycin (Gibco, catalog # 15140163). The cell line used for

(16) Tracking cell-type-specific temporal dynamics in human and mouse brainsCellSeptember 28, 2023Lu Z, Zhang M, Lee J, Sziraki A, Anderson S, Zhang Z, Xu Z, Jiang W, Ge S, Nelson PT, et al.CO2 in high glucose DMEM (Gibco, 11965–118) supplemented with 10% Fetal Bovine Serum (Sigma-Aldrich, F4135) and 1X penicillin-streptomycin (Gibco, 15140–122). Animals The C57BL/6 wild-type mice and 5XFAD transgenic

(17) Human BBB-on-a-chip reveals barrier disruption, endothelial inflammation, and T cell migration under neuroinflammatory conditionsFrontiers in Molecular NeuroscienceSeptember 25, 2023Arya Lekshmi Nair, Linda Groenendijk, Roos Overdevest, Tania M. Fowke, Rumaisha Annida, Orsola Mocellin, Helga E. de Vries, Nienke R. WeversThermo Scientific, 156340) in RPMI 1640 medium (Sigma, R0883) supplemented with 10% fetal bovine serum (FBS, Sigma, F4135), 1% GlutaMAX™ (Gibco, 35050038) and 1% penicillin–streptomycin (Sigma, P4333). THP-1 cell density was

(18) Methods to Quantify the Dynamic Recycling of Plasma Membrane ChannelsBIO-PROTOCOLSeptember 5, 2023Rawad Hodeify, Khaled Machaca16005-1KG-R)Triton X-100 (Sigma, catalog number: T8787-50ML)10% heat-inactivated FBS (Sigma, catalog number: F4135-500ML)FalconTM 15 mL conical centrifuge tubes, polypropyleneFalconTM conical centrifuge tubes, 15 mL

(19) Substrate topographies modulate the secretory activity of human bone marrow mesenchymal stem cellsStem Cell Res TherAugust 21, 2023Heizel Rosado-Galindo, Maribella Domenechexpanded in DMEM high glucose (D6429, Sigma) supplemented with 10% heat-inactivated fetal bovine serum (F4135, Sigma) and 1% penicillin–streptomycin (P4333, Sigma) and maintained at 37℃ and 5% CO2 in a humidified

(20) PFKFB3-Mediated Glycolysis Boosts Fibroblast Activation and Subsequent Kidney FibrosisCellsAugust 17, 2023Yang Q, Huo E, Cai Y, Zhang Z, Dong C, Asara JM, Wei Q.purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM with 10% FBS (F4135, Sigma-Aldrich, St. Louis, MO, USA) in a humidified incubator with 5% CO2 at 37 °C. For testing TGFβ1-induced

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Fetal Bovine Serum from MilliporeSigma

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Fetal Bovine Serum from MilliporeSigma

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